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KMID : 0613820140240040419
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Journal of Life Science
2014 Volume.24 No. 4 p.419 ~ p.427
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Antioxidant Characteristics of Artemisis capillaris Hot-water Extract Using Response Surface Methodology
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Kim Seong-Ho
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Abstract
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In order to examine antioxidative characteristics of Artemisia capillaris response surface methodology was used to optimize the hot water extraction process by analyzing and monitoring the extraction condition. For total phenolic compounds content, the optimal extraction temperature, time and amount of solvent per sample were 94.50℃, 2.06 hr and 25.03 ml/g, respectively. Also, the optimal conditions for electronic donating ability were 91.82℃, 2.90 hr and 20.88 ml/g, respectively. The nitrile scavenging ability (pH 1.2) was optimized using the extraction temperature of 97.36℃, extraction time 2.75 hr and 15.19 ml/g as the amount of solvent per sample. Regression equations of total phenolic compounds content, electron donating ability and nitrile scavenging ability as dependent variable were deduced from each analyzed extraction condition. And finally, their response surfaces were superimposed with the optimal conditions to obtain values for each extraction process factor. The predicted results through superimposing were extraction temperature 90∼95℃, extraction time 2.5∼3.5 hr and amount of solvent per sample 17~24 ml/g.
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KEYWORD
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Antioxidative characteristics, Artemisia capillaris, extraction condition, response surface methodology
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